Additional file 3 of Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression

Additional file 3: Figure S3. Selective sorting of hY4F into EVs inhibits the proliferation, migration, and invasion of lung cancer cells. (A) H1975 cells were incubated with hY4F mimic/NC-overexpressing A549 EVs for 48 h and then the level of hY4F was examined by qPCR. (B) The EdU assay was performed to assess the effect of hY4F-overexpressing A549 EVs on H1975 cell proliferation (at 48 h after treatment). (C) Flow cytometry using Annexin V-FITC/PI staining was performed on H1975 cells incubated with hY4F-enriched A549 EVs to analyze apoptosis (at 48 h after treatment). (D-E) The effect of hY4F-enriched A549 EVs on H1975 migration and invasion were assessed by transwell assay (at 48 h after treatment). Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). NC: negative control.