Additional file 7 of Functional interaction between S100A1 and MDM2 may modulate p53 signaling in normal and malignant endometrial cells

Additional file 7: Figure S7. Changes in proliferation and apoptosis following overexpression of S100A1 in Ishikawa cells. (A) Upper left: two independent clones of stable Ish-S100A1 cells and control cells were seeded at low density. The cell numbers are presented as mean ± SDs. P0, P3, P6, and P9 are 0, 3, 6, and 9 days after cell passage, respectively. Upper right: FACS analyses of stable Ish-S100A1 and control cells at 3 days after seeding (P3). Lower: western blot analysis of the indicated proteins in stable Ish-S100A1 cells and controls following re-stimulation of serum-starved (24 h) cells with 10% serum for the indicated times. The p53 band is indicated by arrows. (B) Upper: stable Ish-S100A1 and control cells were treated with 1 μg/mL Adriamycin (ADR) for the times shown. Daggers indicate the sub-G1 fraction. Lower: the percentages of cells undergoing apoptosis (sub-G1 fractions) were calculated following flow cytometry. C, control (C) Left: after 1 μg/mL ADR treatment, stable Ish-S100A1 and control cells undergoing apoptosis are indicated by arrows. Original magnification, x400. Right: the numbers of apoptotic cells are shown as mean ± SDs. Con, control (D) Western blot analysis of the indicated proteins in total lysates from stable Ish-S100A1 and control cells treated with 1 μg/mL ADR for the times shown. The p53 band is indicated by arrows.