Interaction of phenolic acids with the aryl hydrocarbon receptor (AhR)

Copyright information:Taken from "Antiproliferative and apoptotic effects of selective phenolic acids on T47D human breast cancer cells: potential mechanisms of action"Breast Cancer Research 2003;6(2):R63-R74.Published online 15 Dec 2003PMCID:PMC400651.Copyright © 2004 Kampa et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Interaction of 10M phenolic acids on the AhR against TCDD. Competition of caffeic acid on aryl hydrocarbon binding. Homologous displacement with TCDD given for comparison. Inhibition of basal and TCDD-stimulated CYP1A1 (-dealkylation of ethoxyresorufin [EROD]) activity by 10M caffeic acid. Dose effect of caffeic acid on the (EROD) activity of CYP1A1, after 24 hours of incubation. The time course of the basal and the TCDD-stimulated CYP1A1 transcript, after application of 10M caffeic acid. RT-PCR data were normalized for actin, and divided by the corresponding control values (control = 1 in all cases). PAA, 3,4-dihydroxy-phenylacetic acid.