Additional file 2 of Human placental mesenchymal stromal cells are ciliated and their ciliation is compromised in preeclampsia

Additional file 2: Figure S2. The motility and homing ability is impaired in PE hCV-MSCs as well as their capacity to stimulate motility and MMP2/9 activity in EVT cells. (A) HTR cells and hCV-MSCs from 1st trimester, term and term PE placentas were seeded into each Ibidi chamber. After 8 h, the chambers were removed and the hCV-MSCs started to migrate toward HTR cells. After 4 and 8 h bright-field images were taken for analysis. For fluorescence visualization, the cells were stained with phalloidin (actin filaments, red), p-FAK (focal adhesion marker, green) and DNA (DAPI, blue). (A, left panel) Representatives of the hCV-MSCs at the migrating front after 8 h are shown. Scale: 50 μm. (A, right graph) The length of the cellular protrusions of hCV-MSCs toward HTR cells was quantified, and was presented as scatter plot showing mean ± SEM (n = 180 protrusions, pooled from three independent experiments. (B) Single HTR cells were tracked after the treatment with indicated medium (control medium or medium containing 50% supernatant from hCV-MSCs of 1st trimester, term or term PE placentas) using time-lapse microscopy to analyze their cell motility. The velocity (C, left plot) and accumulated distance (C, right plot) were evaluated for each individual treatment. The results from three experiments are depicted as scatter plots showing mean ± SEM (n = 90 cells). Unpaired Mann-Whitney U test was used for (A and B). ∗p