Biomarker discovery for colon cancer using a 761 gene RT-PCR assay-1

Copyright information:Taken from "Biomarker discovery for colon cancer using a 761 gene RT-PCR assay"http://www.biomedcentral.com/1471-2164/8/279BMC Genomics 2007;8():279-279.Published online 15 Aug 2007PMCID:PMC1994687.ed. Two pools were selected at random to prime separate RT-PCR reactions using RNA from FPE tissue. The 8 GSP pools were then combined to make a single GSP pool that was used to prime one RT-PCR reaction using the same FPE RNA template. The data therefore represents concordance between primings for the subset of gene assays represented with those two GSP pools. Both priming methods were performed twice and the average Cvalues determined for this analysis. The solid line represents the least squares line fit and the dashed line represents the line of concordance.