Characterization of the anti-17βHSD2 antibody

Copyright information:Taken from "Human type 2 17beta-hydroxysteroid dehydrogenase mRNA and protein distribution in placental villi at mid and term pregnancy"http://www.rbej.com/content/5/1/30Reproductive biology and endocrinology : RB&E 2007;5():30-30.Published online 10 Jul 2007PMCID:PMC1947994. Western blot analysis: Cytosol and microsomes extracted from human placental villi were resolved on a 12% SDS-PAGE and transferred on nitrocellulose membrane. Immunodetection was performed using purified rabbit anti-17βHSD2 antibody (), preimmune rabbit serum (), or antibody pre-adsorbed with an excess (10M) of 17βHSD2 synthetic polypeptide (). Specificity of the anti-17βHSD2 antibody was also studied by two-dimensional gel electrophoresis (). Microsomal fraction of placental villi was separated by 2-dimensional isoelectric focusing-SDS-PAGE. Panel D, silver staining. Panel E, immunodetection with the purified anti-17βHSD2 antibody. Position of the circle on panel D corresponds to the location of the circle on panel E. The upper arrow corresponds to a pI value of approximately 8 to 9 and arrow on the right side corresponds to the expected molecular weight.