An effective protocol for analyzing the Anxiolytic Effects of bioactive oligosaccharide and Reverse the Impact of Chronic physical Stress in Mice based on Gut microbiota modulation

· In vivo evaluation - Antistress efficacy in mice Model Animals and maintenance protocol Physical stress induction in mice Weight and feed intake measurement· Behaviour analysis based on stress induction Elevated plus-maze test Open-field test Novel object recognition test· Neurotransmitter - Corti...

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Hauptverfasser: Chelliah, Ramachandran, Park, Se Jin, Oh, Sungyoon, Lee, Eunseok, Daliri, Eric Banan-Mwine, Elahi, Fazle, Park, Chae Rin, Sultan, Ghazala, Madar, Inamul hasan, Oh, Deog-Hwan
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Sprache:eng
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Zusammenfassung:· In vivo evaluation - Antistress efficacy in mice Model Animals and maintenance protocol Physical stress induction in mice Weight and feed intake measurement· Behaviour analysis based on stress induction Elevated plus-maze test Open-field test Novel object recognition test· Neurotransmitter - Corticosterone and serotonin Levels determination· Screening of microbiota modulation based on metagenomics analysisNext-generation sequence (NGS) - Illumina sequence workflowNGS data analysisQuality assessment and sample sequencingMetagenomics Data Analysis Data filtering and Normalization
DOI:10.6084/m9.figshare.18328367