An effective protocol for analyzing the Anxiolytic Effects of bioactive oligosaccharide and Reverse the Impact of Chronic physical Stress in Mice based on Gut microbiota modulation
· In vivo evaluation - Antistress efficacy in mice Model Animals and maintenance protocol Physical stress induction in mice Weight and feed intake measurement· Behaviour analysis based on stress induction Elevated plus-maze test Open-field test Novel object recognition test· Neurotransmitter - Corti...
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Sprache: | eng |
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Zusammenfassung: | · In vivo evaluation - Antistress efficacy in mice Model Animals and maintenance protocol Physical stress induction in mice Weight and feed intake measurement· Behaviour analysis based on stress induction Elevated plus-maze test Open-field test Novel object recognition test· Neurotransmitter - Corticosterone and serotonin Levels determination· Screening of microbiota modulation based on metagenomics analysisNext-generation sequence (NGS) - Illumina sequence workflowNGS data analysisQuality assessment and sample sequencingMetagenomics Data Analysis Data filtering and Normalization |
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DOI: | 10.6084/m9.figshare.18328367 |