D2-mice do not develop glaucoma

Copyright information:Taken from "Absence of glaucoma in DBA/2J mice homozygous for wild-type versions of and "http://www.biomedcentral.com/1471-2156/8/45BMC Genetics 2007;8():45-45.Published online 3 Jul 2007PMCID:PMC1937007. . H&E stained sections of D2-mice at 4.5 mo (), 12 mo () and 18 mo () showing a normal nerve fiber layer (arrows) and no evidence of optic nerve cupping. . H&E section of a 12 months old D2 mouse with severe glaucoma, showing optic nerve cupping (asterisk) and loss of nerve fiber layer (arrows). . PPD staining of the retro-orbital optic nerve from a 4.5 mos (), 12 mos (), 18 mos () D2-mice. These nerves are healthy with only rare degenerating axons, as is typical for nerves of non-glaucomatous mouse strains. . Severe glaucoma in a 12 mos D2 eye. Healthy axons have clear axoplasm, surrounded by darkly stained myelin (arrow). The sick or dying axons stain grey/black (arrowhead). () Frequency of glaucomatous optic nerve damage in D2-mice, using the 3 point grading system (see Methods). No D2-mice show severe glaucoma, and only 3 eyes show moderate damage. Importantly, these moderately affected nerves only just enter this grade (all had less than 10% axon loss), and were not as severely degenerated as the typical D2 moderate nerve.