Additional file 2 of LncRNA MIR205HG accelerates cell proliferation, migration and invasion in hepatoblastoma through the activation of MAPK signaling pathway and PI3K/AKT signaling pathway

Additional file 2. Figure S1 Gain-of-function experiments and in vivo assays for the evaluation of the role of MIR205HG in HB. (A) MIR205HG expression was up-regulated by pcDNA3.1/MIR205HG in THLE-3 cells. (B) EdU assays evaluated the proliferative capacity of THLE-3 cells after MIR205HG was overexpressed. (C-D) Transwell assays were implemented to observe the migration and invasion of THLE-3 cells after the overexpression of MIR205HG. (E) The tumor growth in sh-NC and sh-MIR205HG#1 groups was recorded. (F) The tumor weight was measured in sh-NC and sh-MIR205HG#1 groups. GAPDH and U6 were used as internal references for RT-qPCR. Each experiment was performed in triplicate. Student’s t-test was adopted for statistics. **P