Antisense RNA decreases AP33 gene expression and cytoadherence by -0

Copyright information:Taken from "Antisense RNA decreases AP33 gene expression and cytoadherence by "http://www.biomedcentral.com/1471-2180/7/64BMC Microbiology 2007;7():64-64.Published online 3 Jul 2007PMCID:PMC1929106.parasites. The ethidium bromide (EtBr)-stained band after electrophoresis in 1% agarose is the PCR product of the gene that was amplified using DNA from transfected parasites. As expected, no PCR product was expected from wt organisms, and a predicted product was obtained from the plasmid used directly during PCR as a control (part A, lane labeled C). Part B gives bands after electrophoresis as in part A showing RT-PCR products for the S (panel a) and AS transcript (panel b). The RT-PCR product for and are presented in panels c and d, respectively. Part C illustrates the quantitation of the transcript bands in part B (panel a) for the transcript in AS-transfected trichomonads compared to S-transfected and wt parasites. The bar graph shows the relative amounts of RT-PCR products for . The amount of wild type transcript was normalized 100%. Quantification was done following Scion image β program.