Biliary sterol, phospholipid and bile salt analyses

Copyright information:Taken from "A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol"BMC Medicine 2004;2():5-5.Published online 24 Mar 2004PMCID:PMC394351.Copyright © 2004 Klett et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Bile salt, sterol and phospholipid contents from male +/+ (n = 8), +/- (n = 7), and -/- (n = 5) mice were examined as described in Methods. Bile salt secretion following a 10 minute collection was lower in +/- and -/- mice compared to wild type (panel a), but these differences were not statistically significant. Biliary sterol and phospholipid were significantly reduced in the -/- mice compared to the wild-type (panels b and c). *< 0.05. The lower panel shows biliary bile salt, sterol and phospholipid secretion rates from female +/+ (n = 5), +/- (n = 4), and -/- (n = 3) mice measured following 90-minute bile salt depletion followed by stepwise TUDC infusion as described in the text. Bars represent phase TUDC infusion rates. No differences were observed in the ability of the -/- mice to secrete bile salts (panel d), but there is a marked inability of the knockout mice to secrete sterols (panel e) and a trend towards a reduced ability to secrete phospholipid (panel f) compared to wild type.