Sorting efficiency with the COPAS machine

Copyright information:Taken from "A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode "Nucleic Acids Research 2006;35(2):e11-e11.Published online 12 Dec 2006PMCID:PMC1903375.© 2006 The Author(s). () Sorting at the F1 stage. Bars represent the average for two independent trials of 96 wells, with error bars showing the standard deviation. The percentage of worms expressing GFP was calculated counting only those wells that contained a single worm; 100% of the singled worms had GFP expression in the pharynx. () Sorting at the F4 stage. Data are shown for a typical 96-well plate.