Additional file 3 of Modeling ischemic stroke in a triculture neurovascular unit on-a-chip

Additional file 3. Neurons in the NVU model show spontaneous electrophysiological activity. A fluorescent calcium indicator was loaded into the cells of the NVU on-a-chip model at day 14 of culture. Cells show an increase in fluorescence upon calcium influx into the cell, which is associated with neuronal firing. Calcium fluctuations were captured at 0.5 Hz using a fluorescent microscope. (a) Images depict low (dark colors) and high (light colors) intracellular calcium and show a changing pattern in cells over time. (b) Fluctuations in calcium signal were plotted over time for four randomly selected active neurons.