Additional file 4 of Immunoprofiling of early, untreated rheumatoid arthritis using mass cytometry reveals an activated basophil subset inversely linked to ACPA status

Additional file 4 of Immunoprofiling of early, untreated rheumatoid arthritis using mass cytometry reveals an activated basophil subset inversely linked to ACPA status

Additional file 4: Supplementary Figure 2: Gating strategy for data cleanup of Flow Cytometry files. Flow Cytometry files were unmixed within the CYTEK acquisition software based on single stained controls. Forward and Side-scatter Area are used to remove most debris. Single cells are defined throug...

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Hauptverfasser: Koppejan, H., Hameetman, M., Beyrend, G., van Unen, V., Kwekkeboom, J. C., van der Helm-van Mil, A. H., Toes, R. E. M., van Gaalen, F. A.
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Sprache:eng
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FOS: Clinical medicine
Immunology
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Zusammenfassung:Additional file 4: Supplementary Figure 2: Gating strategy for data cleanup of Flow Cytometry files. Flow Cytometry files were unmixed within the CYTEK acquisition software based on single stained controls. Forward and Side-scatter Area are used to remove most debris. Single cells are defined through combination of Height and Area, both for Forward and Side-scatter. Live cells are defined as ZOMBIE-yellow negative. All samples are pre-gated for CD45+ and exported as new FCS file, similar to MC data processing.
DOI:10.6084/m9.figshare.16907551