Additional file 15 of The mitochondrially-localized nucleoside diphosphate kinase D (NME4) is a novel metastasis suppressor

Additional file 15: Fig. S9. NDPK-D protein expression and mitochondrial localization in MDA-MB-231 clones. A) Immunoblot detection of NDPK-D from MDA-MB-231 cells stably transfected with empty pcDNA4TO (CTR) or constructs for expression of NDPK-D WT, BD or KD. Alpha-tubulin was used as loading control. B) MDA-MB-231 clones stably transfected with empty vector (CTR), or expressing NDPK-D WT or mutants BD or KD, showing labeling of mitochondrion-selective dye MitoTracker Red CMXRos (red) and immunolabeled NDPK-D (green). Mitochondrial network details are indicated by faint line boxes magnified in bold line boxes. Scale bar, 10 μm.