Brown Nat Protocols Fig 3b

Figure 3 | Comparison of chromatin structure and TAD shapes. Super-resolution 3D-SIM imaging of human RPE-1 cells after a control IF treatment or indicated FISH protocol using a KIF23 TAD-specific probe. b, Exemplary TAD FISH signals selected from several cells highlight more defined and distinct edges of the TAD signal after RASER detection compared to 4 min, 4 min+dry (bottom row of middle section) and 30 min+dry heat denaturation. Images show false colour representations of maximum intensity projections. Scale bar, 1 µm.