Brown Nat Protocols Fig 2b

2b, Comparison of single-strandedness and nuclear integrity. Single-stranded DNA detected by antibody in example HeLa and C127 cells after simple immunofluorescence (IF control) or the three FISH methods, 4 min, 4 min+dry, RASER. Upper panels show the ssDNA antibody labelling signal (green/white). Scale bar, 5 µm. Lower panels show an expanded view of the nuclear periphery of the same cells with the ssDNA signal (green) against a DAPI counterstain (purple). The disruption to nuclear integrity in the heat-denatured samples is evident. Scale bar, 1 µm.