ER-α expression in reconstituted mammary glands

Copyright information:Taken from "Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the host microenvironment"http://breast-cancer-research.com/content/9/2/R22Breast Cancer Research 2007;9(2):R22-R22.Published online 6 Mar 2007PMCID:PMC1868922. Epithelial mammary cells of BALB/c or C57BL/6 mice were purified and implanted into cleared fat pads of untreated /mice (as explained in Materials and methods). After 1 month, mammary glands were excised and fixed for histological evaluation. ER-α was evaluated using MC 20 Santa Cruz antibody and PR-A (not shown) using C-20 Santa Cruz antibody (bar: 45 μm). The number of stained cells is expressed as the percentage of stained cells among epithelial cells in 400× fields of every slide. ER, estrogen receptor; PR, progesterone receptor.