Toeprint caused by L20 binding to the leader mRNA

Copyright information:Taken from "Ribosomal protein L20 controls expression of the operon via a transcription attenuation mechanism"Nucleic Acids Research 2007;35(5):1578-1588.Published online 8 Feb 2007PMCID:PMC1865079.© 2007 The Author(s). Oligonucleotide HP697 was extended on a wild type and ‘C’ mutant (CC → GG, see ) transcript of the leader region. Where indicated the transcript was incubated with the r-proteins L20 or L17 prior to the extension reaction. The reverse transcriptase stop caused by L20 bound to the transcript is indicated by an arrow; it corresponds to a primer extension arrest at uridine residue 72 (see ). The sequence ladder was generated with the same primer HP697, the encircled nucleotide indicates the arrest position on the cDNA.