Additional file 5 of Circular RNA circ_001422 promotes the progression and metastasis of osteosarcoma via the miR-195-5p/FGF2/PI3K/Akt axis

Additional file 5: Figure S4. Overexpression of miR-195-5p rescues the OE-circ_001422-mediated promotive effects on OS cell proliferation and metastasis. a & b. OS cells were transfected with OE-circ_001422-NC or OE-circ_001422 or were cotransfected with OE-circ_001422 and the miR-195-5p mimic. EdU incorporation (scale bar, 100 μm) and colony formation assays were performed to evaluate the proliferation ability of OS cells in each group. c & d. Flow cytometry was performed to evaluate the effect of miR-195-5p overexpression on the OE-circ_001422-mediated effects on the cell cycle distribution and apoptosis. e & f. Transwell migration and Matrigel invasion assays were performed to evaluate the effect of miR-195-5p overexpression on the OE-circ_001422-mediated enhancement of the migration and invasion abilities. Scale bar, 100 μm. g. The protein levels of CCND1, CDK4, cleaved CASP3, BAX, BCL2, E-cadherin, N-cadherin and Vimentin were analyzed by western blotting and normalized to the level of GAPDH. n.s., not significant; *P