Additional file 4 of Human umbilical cord-derived mesenchymal stem cells improve the function of liver in rats with acute-on-chronic liver failure via downregulating Notch and Stat1/Stat3 signaling

Additional file 4: Figure S3. The expression of Notch signaling pathway related genes and Stat1/Stat3 signaling molecules in ACLI and ACLF rats. Total RNA and protein were extracted from the liver tissues from rats with ACLI or ACLF transplanted with hUC-MSCs or 0.9% sodium chloride as a control (n = 3). The mRNA expression levels of Notch1, Hes1, and P21 in ACLI rats were detected by quantitative PCR (a). Total protein was subjected to western blotting analysis. Relative protein expression of Stat1, pStat1, Stat3 and pStat3 in ACLF rats were quantitated (b). Relative protein expression of Stat1, pStat1, Stat3, pStat3, c-Myc, Bcl2 and CyclinD1 in ACLI rats were quantitated (c-f). Data were presented as the mean ± SD. *P < 0.05.