Effect of VP16 and GA on topoIIα–DNA complexes

Copyright information:Taken from "Inhibition of Hsp90 acts synergistically with topoisomerase II poisons to increase the apoptotic killing of cells due to an increase in topoisomerase II mediated DNA damage"Nucleic Acids Research 2006;34(4):1148-1157.Published online 16 Feb 2006PMCID:PMC1373695.© The Author 2006. Published by Oxford University Press. All rights reserved Cells were treated with 2 µM VP16, 500 nM GA and the combination of the two for 1 h. Cells were collected and lysed with 1% sarkosyl, loaded onto pre-formed CsCl step gradients and centrifuged at 30 000 r.p.m. for 24 h. Fractions were collected and applied onto nitrocellulose membranes, which were immunoblotted with anti-human topoisomerase IIα antibodies. Lanes 13–18 not shown.