RecJ cleaves the terminal nucleotides on 5′ tails

Copyright information:Taken from "RecJ exonuclease: substrates, products and interaction with SSB"Nucleic Acids Research 2006;34(4):1084-1091.Published online 18 Feb 2006PMCID:PMC1373692.© The Author 2006. Published by Oxford University Press. All rights reserved DNA substrates with 5′ single-stranded tails of 5, 6, 7, 8 and 9 nt in length were subjected to conditions where RecJ binding only could be observed [(B), no Mg] and conditions where nuclease activity could be detected [(C), 10 mM MgCl] by loss of radioactive label at the 5′ end of the single-stranded tail. The 5 nM RecJ protein was combined with 5 nM substrate in binding reactions whereas 12.5 nM protein was combined with 5 nM substrate in cleavage reactions. Control reactions (A) contained no protein.