Additional file 1 of Transcription factor signal transducer and activator of transcription 6 (STAT6) is an inhibitory factor for adult myogenesis

Additional file 1: Figure S1. Differentiation index in STAT6-overexpressed and STAT6-inhibited cells. (A) Differentiation index in the Ad-Ctrl and Ad-STAT6 treatments. n = 5. *p < .05 vs. Ad-Ctrl. Images in Fig. 2 were used for analysis. (B) Differentiation index in the Ctrl and shSTAT6 treatments. n = 5. *p < .05 vs. Ctrl. Images in Fig. 3 were used for analysis. Data are presented as mean ± SD. Figure S2. Proliferation of STAT6-overexpressed myoblasts. (A) Representative bright-field images of myoblasts in Ad-Ctrl and Ad-STAT6 cells. Scale bar = 50 μm. (B) Absorbance at 450 nm in Ad-Ctrl and Ad-STAT6 cells using a CCK cell counting kit. (C) Proliferation rate in Ad-Ctrl and Ad-STAT6 cells. n = 6. Data are presented as mean ± SD. Figure S3. Pax7-positive cells in regenerating TA muscle of WT and STAT6-KO mice. (A) Representative images in CTX-injured TA muscle. Scale bar = 50 μm. (B) Quantification of pax7+ cells per myofiber. n = 5. Data are presented as mean ± SD. Supplementary Table S1. Primer sequences for QRT-PCR.