Additional file 2 of High-fat diet impairs duodenal barrier function and elicits glia-dependent changes along the gut-brain axis that are required for anxiogenic and depressive-like behaviors

Additional file 2 Representative images of duodenal cross-sections stained before starting the diet protocol. Representative images of duodenal cross-sections stained by (A) hematoxylin and eosin or immunolabeled for (C) CD45 (brown) or (E) Dapi (blue), ZO-1 (green), and occludin (red) before starting the diet protocol. (B) Relative total histological damage score, (D) average number of CD45+ cells/mm2. Data were analyzed by 2-way ANOVA or 1-way ANOVA and Dunnettpost-hoc. Results are expressed as cumulative histological damage score ± SEM or the average relative fluorescence units (RFU) ± SEM per area unit of n assessments. Scale bars = 10 and 30 μm.