Additional file 1 of High-fat diet impairs duodenal barrier function and elicits glia-dependent changes along the gut-brain axis that are required for anxiogenic and depressive-like behaviors

Additional file 1 HFD increases body weight during 20 weeks of diet protocol. (A) Schematic representation of the experimental protocol with time schedule for immunohistochemistry assessments in the enteric and central nervous systems and behavioural tests. Mice were fed with a standard diet (SD) or 72% high-fat diet (HFD) for 20 weeks, alone or with a daily IP of 10 μmol/Kg fluorocitrate (FC) to investigate the enteric glia involvement in HFD-induced neuropathology. (B) Weekly body weight gain exhibited by standard diet (SD) and high-fat diet (HFD) mice during the 20 weeks of diet protocol. Data were analyzed by 2-way ANOVA and Dunnettpost-hoc. Results are expressed as average weights than their SD counterparts.