Additional file 3 of Loss of EZH2-like or SU(VAR)3–9-like proteins causes simultaneous perturbations in H3K27 and H3K9 tri-methylation and associated developmental defects in the fungus Podospora anserina

Additional file 3: Figure S3. A Heatmap of Spearman’s correlation coefficient comparison: clustering analysis of histone marks in wild-type background (WT). 2e and 3d are the two WT strains used for this study. They are issued from two spores from the same WT cross. Mock = IP performed with GFP antibody in absence of GFP tag in P. anserina’s genome (see “Methods”). Raw data are given in Additional file 25: Table S7. B H3K27me3, H3K4me3 and H3K9me3 proportion on P. anserina chromosomes in the WT, ΔPaKmt1, ΔPaKmt6 and ΔPaHP1 mutant strains. Plot showing the percentage of each chromosome covered with H3K4me3 (green), H3K9me3 (red) and H3K27me3 (blue). The coverage is the sum of all MACS2-predicted peak sizes. C Normalized ChIP-seq data representation for all marks on the seven P. anserina chromosomes for all conditions. ChIP-seq patterns display histone modification coverage and MACS2 detected peaks. D Domainogram representations for all marks on the seven P. anserina chromosomes for all conditions. Domainograms show significance of enrichment of H3K4me3, H3K9me3, H3K27me3 marks in windows of varying size. Color-coding of p-value is indicated (top).