Synthesis of the tC phosphoramidite

Copyright information:Taken from "Fluorescent properties of DNA base analogue tC upon incorporation into DNA — negligible influence of neighbouring bases on fluorescence quantum yield"Nucleic Acids Research 2005;33(16):5019-5025.Published online 7 Sep 2005PMCID:PMC1201328.© The Author 2005. Published by Oxford University Press. All rights reserved Reagents and conditions: (a) (i) 1.1 eq. NaH in DMF, RT, (ii) 1.1 eq. 2-deoxy-3,5-di--p-toluoyl-α--erythro-pentofuranosyl chloride, 2 h, RT; (b) 1 eq. NaOMe in methanol, RT, 14% from 1; (c) 1.1 eq. 4,4′-dimethoxytrityl chloride in pyridine, 2 h, RT, 70%; (d) 1.5 eq. 2-cyanoethyl-,-diisopropylchlorophosphoramidite, 2.5 eq. diisopropylethylamine in anhydrous tetranhydrofuran, 30 min, RT, 94%.