Additional file 1 of A simple and affordable method for estimating the fluid volume a mosquito sucks using food dyes

Additional file 1: Figure S1. Equipment for membrane feeding used in this study. (a) Each glass feeder covered with stretched parafilm was placed on a paper cup containing approximately thirty female mosquitoes at 5–7 days after eclosion. After applying ATP solution into each feeder, 42 °C water from a hot water bath coupled with a water pump was circulated through rubber tubes connected to each feeder. (b) Magnified view of a paper cup with a glass feeder. Note that mosquitoes swarm around the feeder and suck the green colored solution.