Metabarcoding lemur diet dataset two - ZBJ COI primer set

These datasets are associated with the paper titled "Exploratory analysis reveals arthropod consumption in ten lemur species using DNA metabarcoding" accepted in the American Journal of Primatology. Each dataset contains paired .fastq files for 192 samples These data were generated by sequencing faecal samples from lemurs (sampled following all correct protocols - see paper) and detecting arthropod DNA in their diet. We generated the sequences in this dataset by extracting total DNA from faecal samples collected from various lemur species. PCRs were then conducted using two primer sets. Dataset one contains the amplicons generated using primers we coded "Ber-Luthien", sequences from Cuff et al 2020 (Ecological Entomology, doi.org/10.1111/een.12957) which targets a 312bp region of arthropod COI Dataset two contains the amplicons generated using primers we coded "ZBJ", sequences from Zeale et al 2010 (Molecular ecology resources, doi.org/10.1111/j.1755-0998.2010.02920.x) which targets a different region of the arthropod COI gene and generates a shorter fragment (157bp)All amplicons were pooled and sequencing using Illumina MiSeq (250bp PE)The sample ID (AKR#) and the corresponding lemur species from which the lemur faecal sample was analysed can be found in the file SampleInfo.xlsxData was analysed using the mBRAVE platform (www.mbrave.net)