Additional file 7 of Comparative Analysis of Genome of Ehrlichia sp. HF, a Model Bacterium to Study Fatal Human Ehrlichiosis

Additional file 7: Figure S2. Gene Structures of Ehrlichia sp. HF Type IV Secretion System. Ehrlichia sp. HF encodes a Type IV secretion system. These virB/D genes are split into three major operons: virB2/4, virB3/4/6virB3/4/6, virB8/9/10/11/D4, and three separate loci: virB7 and duplicated virB8-2 and virB9-2. virB2 genes are duplicated into 5 copies, whereas virB6 into 4 copies. Genes encoding virB1 and virB5 are not present in HF genome. Note: Due to the short protein length and low homology, virB7 was not annotated as an ORF by NCBI automated annotation pipeline. However, by TBLASTN using A. marginale VirB7 protein sequence [121] against the entire HF genome sequence, a putative virB7 gene was identified and manual curated.