Additional file 3 of CircCDKN2B-AS1 interacts with IMP3 to stabilize hexokinase 2 mRNA and facilitate cervical squamous cell carcinoma aerobic glycolysis progression

Additional file 3: Fig. S1. Identification of circCDKN2B-AS1, circFNACB, circEPSTI1 and circOBSL1. (A) Sequencing analysis of products amplified by the divergent primers showed the junction in circFNACB. (B) Sequencing analysis of products amplified by the divergent primers showed the junction in circEPSTI1. (C) Sequencing analysis of products amplified by the divergent primers showed the junction in circOBSL1. (D) Linear and circular isoforms of CDKN2B-AS1 were amplified from cDNA or gDNA from SiHa cells with the convergent primers and divergent primers, respectively. (E) The backsplicing point-specific probe was used in Northern blot analysis to detect endogenous circCDKN2B-AS1 in cervical cancer cell lines. Left lane: RNA molecular weight markers (2661, 1821, and 1517); right lane: circCDKN2B-AS1-specific probe.