Additional file 2 of Targeted expression profiling reveals distinct stages of early canine fibroblast reprogramming are regulated by 2-oxoglutarate hydroxylases

Additional file 2: Supplemental Figure 2. Maturity of donor tissue influences primary canine fibroblast proliferation and the effect of L-ascorbic acid or retinoic acid on TET paralogue transcript level. (A) Isotype controls for direct immunofluorescence staining of canine fetal fibroblast (cFF) cultures, related to (i) CD44-PE or (ii) CD90-PE staining experiments in Fig. 1A. (B) Fold-increase in total cell number over five days of adherent canine adult fibroblasts (cAF) and cFFs. (C) Population doubling time intervals calculated from linear growth phase in cAF and cFF. (D) Ratio of 5-hydroxymethylcytosine (5-hmC) to 5-methylcytosine (5-mC) in media containing AA/RA or vehicle diluent. Data are presented as mean ± standard error, n = 4. Relative transcript abundances for (E) cTET1, (F) cTET2 and (G) cTET3 in canine dermal fibroblasts cultured in atmospheric oxygen and exposed to various concentrations of AA and/or RA, n = 3. (H) Representative phase-contrast and fluorescent micrographs of canine dermal fibroblast transduced with emGFP control Sendai vector at various multiplicity of infection (MOI). (I) Summarization of the percent emGFP-positive cells at each MOI determined by flow cytometry. Data are presented as mean n = 2. Means annotated with different letters are considered significantly different by one-way or two-way analysis of variance and Tukey’s honestly squared difference test, P