Video S1 from Trypanosoma cruzi amastigotes that persist in the colon during chronic stage murine infections have a reduced replication rate

3-D video projection and rotation after Z-stack capture of a Vero cell 5 days post-infection with T. cruzi CLBr-Luc::Scarlet (red) (see also figure 1d). Infective trypomastigotes had been pre-incubated with 10 µM CTV (Methods). The asynchronous parasite population contains amastigotes displaying different sizes and shapes suggesting variability in their cell and/or developmental cycles. Within individual parasites, there was considerable heterogeneity in the intensity and location of CTV-staining. Similarly, in the host cell, CTV is widely dispersed (blue), frequently sequestered in vesicle-like structures that can be mistaken for amastigotes unless co-localising red fluorescence is confirmed. N=host cell nucleus. Size bars = 20 µm