Additional file 2 of Pathogenic effects of inhibition of mTORC1/STAT3 axis facilitates Staphylococcus aureus-induced pyroptosis in human macrophages

Additional file 1: Figure S1. PMA-induced differentiation of THP-1 to macrophages and further induction by LPS and IFN-γ into M1-polarized macrophages. THP-1 cells were treated with PMA (100 ng/mL) for 24 h to differentiate into Mϕ -polarized macrophages and then stimulated with IFN-γ (2.5 ng/mL) and LPS (100 ng/mL) to polarize them into classical M1 macrophages. (a) THP-1 and THP-1-derived macrophages and Mϕ and M1 macrophages. Scale bars represent 100 μm. (b) TFN-α and IL-1β in culture medium were determined by ELISA. M1 macrophages had more TFN-α and IL-1β than Mϕ macrophages. **p