Antibody and flow cytometry data following inoculation of rhesus macaques with SARS-CoV-2

This data record consists of a single dataset, Iyer Source data.xlsx, in .xlsx file format. The file includes all the antibody and flow cytometry data supporting the figures and supplementary figures in the related article. The file consists of 11 tabs labelled Figure 1, Figure 2, Figure 3, Figure 4, Figure 5, Figure 6, Figure 7, S1, S2, S3 and S5. Study aims and methodology: CD4 T follicular helper (Tfh) cells are important for the generation of durable and specific humoral protection against viral infections. The degree to which SARS-CoV-2 infection generates Tfh cells and stimulates the germinal center (GC) response is an important question as we investigate vaccine options for the current pandemic. Because healthy rhesus macaques infected with SARS-CoV-2 resist immediate re-challenge with the virus, the authors hypothesized that understanding the CD4 Tfh and GC response following exposure to SARS-CoV-2 will provide a framework for understanding immune mechanisms of protection. The authors tested this hypothesis in the setting of a study designed to examine the therapeutic efficacy of convalescent plasma infusion in curbing a nascent infection. They conducted a comprehensive immune analysis in a controlled animal model of mild diseases, in order to add to our understanding of immune responses to SARS-CoV-2. Eight colony-bred Indian origin rhesus macaques (Macaca mulatta) were housed at the California National Primate Research Center and maintained in accordance with American Association for Accreditation of Laboratory Animal Care guidelines and Animal Welfare Act/Guide. The study received ethical approval by the Institutional Animal Care and Use Committee at UC Davis and research personnel and animal staff complied with all relevant ethical regulations for animal testing and research. The following are described in more detail in the related article: Virus and inoculations, Convalescent plasma and infusions, Specimen collection and processing, Activation induced Marker (AIM) assay, vRNA quantitation by quantitative real time polymerase chain reaction (qRT-PCR), Serum cytokines, Flow cytometry and immunofluorescent staining, Binding antibody multiplex assay (BAMA) for IgG and IgM antibodies to S1, S2 and N proteins, ELISA for SARS-specific IgA and antibodies to RBD, Pseudovirus neutralization assay, and statistical analysis.