Additional file 5 of JARID1B promotes colorectal cancer proliferation and Wnt/β-catenin signaling via decreasing CDX2 level

Figure S3. JARID1B overexpression promoted CRC cells proliferation by inhibiting CDX2 expression. a Western blot detected JARID1B, β-catenin, c-MYC and cyclinD1expression in LOVO cells transfected with Vector or Flag-JARID1B. b The total and nuclear protein levels of β-catenin were assessed by western blotting in LOVO cells transfected with Vector or Flag-JARID1B. c The effect of JARID1B overexpression on Wnt/β-catenin pathway was detected by TOP-Flash luciferase reporter assay. d Western blotting analysis showed downregulation of β-catenin attenuated the increased expression of β-catenin, c-MYC and cyclinD1 in LOVO-JARID1B cells. e TOP-Flash luciferase reporter assay showing that β-catenin knockdown rescued the increased Wnt/β-catenin pathway activity of LOVO-JARID1B cells. g, h Edu assay results showed that β-catenin knockdown significantly inhibited the increase of cells proliferation in LOVO-JARID1B cells. *p