Additional file 4 of Pancreatic and intestinal endocrine cells in zebrafish share common transcriptomic signatures and regulatory programmes

Additional file 4: Figure S3. Expression of vipb and adcyap1a transcripts in zebrafish EECs. Confocal images of the zebrafish gut from larvae stained by double fluorescent in situ hybridization (FISH) using the enteric neurone marker phox2b (green) and the vipb or adcyap1a probes (red). (A) general vue of the gut showing co-localisation of vipb and phox2b in ENs at the level of anterior intestine (upper left part of the image) and vipb+ EECs in the posterior intestine (bottom right of image). (B) higher magnification of the gut showing three vipb+ phox2b+ ENs and two vipb+ EECs. (C) adcyap1a+ cells are distinct from phox2b+ ENs which are located outside the intestinal epithelium (Dapi staining in blue).