Additional file 8 of Trans-synaptic and retrograde axonal spread of Lewy pathology following pre-formed fibril injection in an in vivo A53T alpha-synuclein mouse model of synucleinopathy

Additional file 8: Figure S5. A53T SynGFP aggregates appear first in presumed axons (Video). a Video showing in vivo multiphoton 3D projection of serial images of the same neuron at progressive time points after PFF injection. The orientation of the neuron in the video can be viewed at 0:00:03. At this timepoint the pial surface is at the top of the image and the axon can be seen pointing down towards the deeper cortical layers (still image shown in Fig. 5). A53T SynGFP aggregates (increased GFP intensity shown in green) form first in the axon and predict formation of a somatic inclusion in this neuron.