Autoradiograph showing the outcome of the MTR kinase assay

Copyright information:Taken from "MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis"BMC Microbiology 2001;1():15-15.Published online 8 Aug 2001PMCID:PMC55331.Copyright © 2001 Sekowska et al; licensee BioMed Central Ltd. Verbatim copying and redistribution of this article are permitted in any medium for any non-commercial purpose, provided this notice is preserved along with the article's original URL. For commercial use, contact info@biomedcentral.com The assay was carried out for 90 min at 37°C (see Materials and Methods). Lane 1 corresponds to [•P]ATP as standard; lanes 2, 4 and 6: no MTR was added for the reactions with cell-free extracts of wild type, BFS1850 and BFS1850 grown with IPTG, respectively; lanes 3, 5 and 7: reaction performed in the presence of MTR and cell-free extracts of wild type, BFS1850 and BFS1850 grown with IPTG, respectively.