Additional file 6 of Microglia depletion diminishes key elements of the leukotriene pathway in the brain of Alzheimer’s Disease mice

Additional file 6: Supplementary Figure 6. To confirm our data on the reduction of 5-Lox staining in cortical neurons after microglia ablation, we performed an additional staining with the mouse anti-5-Lox antibody (monoclonal 5-Lox antibody from BD Biosciences #610694), the same used for human tissue. With this antibody 5-Lox immunoreactivity was exclusively found in neurons and did not co-localize with Iba1 positive cells in all groups (inserts). However, most interestingly the 5-Lox staining was again reduced in neurons upon microglia ablation. Dapi was used as nucleus stain. Scale: 20 μm (both).