Additional file 4 of Microglia depletion diminishes key elements of the leukotriene pathway in the brain of Alzheimer’s Disease mice

Additional file 4: Supplementary Figure 4. Immunohistochemical analysis of 5-Lox expression in the cortex. We verified 5-Lox immunostaining in neurons and microglia cells using a monoclonal 5-Lox antibody. 5-Lox (red, monoclonal 5-Lox antibody from Abcam #ab169755) was primarily expressed in neurons but did also co-localize with some Iba1 (white) positive cells (arrows and inserts). Most interestingly, overall the 5-Lox staining was reduced upon microglia ablation using CSF1R inhibitor PLX5622. Remaining microglia in PLX5622 treated mice displayed altered cell morphology. Dapi was used as nucleus stain. Scale: 20 μm and 10 μm inserts.