Additional file 1 of Accumulation of blood-circulating PD-L1-expressing M-MDSCs and monocytes/macrophages in pretreatment ovarian cancer patients is associated with soluble PD-L1

Additional file 1: Fig. S1. Analysis of monocytic myeloid-derived suppressor cells (M-MDSCs) and monocytes/macrophages (MO/MA) as well as programmed death-ligand 1 (PD-L1)-expressing M-MDSCs and MO/MA in ovarian cancer (OC). Mononuclear cells (MCs) from the blood (n = 43), ascites (n = 26) and tumour tissue (n = 29) of OC patients were analyzed. MCs from the blood of healthy women (n = 15) were also examined. Analysis was performed using flow cytometry. MCs were stained for PD-L1+M-MDSCs and MO/MA using fluorochrome-labeled monoclonal antibodies (mAb) against HLA-DR, CD14 and PD-L1(CD274). Representative dot plots from the blood sample of OC patient of HLA-DR−/lowCD14+ M-MDSCs, HLA-DR+CD14+ MO/MA, PD-L1-expressing M-MDSC and PD-L1-expressing MO/MA are shown.