Additional file 5 of RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases

Additional file 5: Figure S5. Nuclear stress body (NSB) formation during RBM45 and SatIII knockdown. HEK293 cells were transfected with siRNAs targeting RBM45, SatIII, or off-target scrambled siRNAs (control). NSB formation was then assessed by immunocytochemistry following treatment with 1 mM sodium arsenite for 1 h. (a) Effect of off-target, scrambled siRNA (control) on NSB formation. Cells transfected with control siRNAs readily form SAFB, RBM45, and HSF1-positive NSBs following treatment with sodium arsenite. (b) Cells transfected with siRNAs targeting RBM45 show reduced levels of RBM45, but readily form SAFB and HSF1-positive NSBs following cellular stress. (c) Knockdown of SatIII leads to a loss of NSB formation during cellular stress as indicated by the loss of SAFB, RBM45, and HSF1-positive NSBs in cells transfected with SatIII targeting siRNAs. For all images, scale bar = 5 μm.