Additional file 3 of Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen

Additional file 3 of Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen

Additional file 3: Table S2. List of scaffold variants, tRNA, and sgRNA cassette. (a) Programmed scaffold variants used in this study. (b) tRNA sequences for gene knockout experiments. (c) Dual-sgRNA expression cassette for Jurkat TCR activation experiment. (d) Design for multiplexed gRNAs expressio...

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Bibliographische Detailangaben
Hauptverfasser: Song, Qingkai, Ni, Ke, Liu, Min, Li, Yini, Wang, Lixia, Wang, Yingying, Liu, Yingzheng, Yu, Zhenxing, Qi, Yinyao, Lu, Zhike, Ma, Lijia
Format: Dataset
Sprache:eng
Schlagworte:
FOS: Biological sciences
Genetics
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Zusammenfassung:Additional file 3: Table S2. List of scaffold variants, tRNA, and sgRNA cassette. (a) Programmed scaffold variants used in this study. (b) tRNA sequences for gene knockout experiments. (c) Dual-sgRNA expression cassette for Jurkat TCR activation experiment. (d) Design for multiplexed gRNAs expression cassette in the activation experiment.
DOI:10.6084/m9.figshare.12452297