Additional file 2 of Cloning of Hynobius lichenatus (Tohoku hynobiid salamander) p53 and analysis of its expression in response to radiation

Additional file 2 of Cloning of Hynobius lichenatus (Tohoku hynobiid salamander) p53 and analysis of its expression in response to radiation

Additional file 2. Immunoprecipitation and western blot analysis of hyp53-HA transiently expressed in mammalian cells. HEK293 cells were transiently transfected with or without phyp53-HA for 2 days. Cell lysates were immunoprecipitated (IP) with anti-HA and western blotted with anti-human p53 (hp53)...

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Hauptverfasser: Kamada, Toshiki, Une, Yumi, Matsui, Kumi, Fuma, Shoichi, Ikeda, Teruo, Okamoto, Mariko
Format: Video
Sprache:eng
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FOS: Biological sciences
Genetics
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Zusammenfassung:Additional file 2. Immunoprecipitation and western blot analysis of hyp53-HA transiently expressed in mammalian cells. HEK293 cells were transiently transfected with or without phyp53-HA for 2 days. Cell lysates were immunoprecipitated (IP) with anti-HA and western blotted with anti-human p53 (hp53) antibody. “Input” indicates the input protein lysate.
DOI:10.6084/m9.figshare.12344888