Additional file 9 of Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Additional file 9: Figure S9. The editing system of gma-miR398c gene in soybean hairy root by CRISPR/Cas9. (A) Soybean precursor miR398c stem-loop structure and sgRNA information. (B) T7E1 enzyme digestion sgRNA-1 and sgRNA-2 editing efficiency. Lanes WT and WT*, undigested and digested wild-type controls, respectively. The red arrowhead indicates the digested bands. The numbers at the bottom of the gels indicate mutation frequencies measured according to band intensities. M, DL2000 ladder DNA marker. (C) Cloning and sequencing of the digested bands. sgRNA-1 and sgRNA-2 target site editing information.