() Determination of the polarity of Sso MCM helicase activity

Copyright information:Taken from "Archaeal MCM has separable processivity, substrate choice and helicase domains"Nucleic Acids Research 2007;35(3):988-998.Published online 26 Jan 2007PMCID:PMC1807962.© 2007 The Author(s). 25, 50 or 100 ng of Sso MCM or 100 ng of Mth MCM were incubated with substrates designed to have 3′, 5 or 3′ and 5′ single–stranded extensions. The positions of substrate (S) and product (P) are indicated. () B shows the helicase activity of the different deletion constructs at various concentrations, N-half and HTH are omitted as no activity was detected. () C compares the activity of the AAA+ core with an equivalent construct possessing a K > A mutation in the Walker A motif.