Supplementary Figure 3.eps

Supplementary Figure 3 LSD1 inhibits degradation of HIF1α protein to up-regulate its expression. A, The protein expression of LSD1 and HIF1α in BHP5-16 cells treated with 100 μM CHX measured by western blot analysis. B, The protein expression of LSD1 and HIF1α in BHP5-16 cells treated with 40 μM MG132 (proteasome inhibitor) measured by western blot analysis. C, The protein expression of LSD1 and HIF1α in BCPAP cells treated with 100 μM CHX measured by western blot analysis. D, The protein expression of LSD1 and HIF1α in BCPAP cells treated with 40 μM MG132 (proteasome inhibitor) measured by western blot analysis. E, The protein expression of LSD1 and HIF1α in BHP2-7 cells treated with 100 μM CHX measured by western blot analysis. F, The protein expression of LSD1 and HIF1α in BHP2-7 cells treated with 40 μM MG132 (proteasome inhibitor) measured by western blot analysis. The data in the figure were all measurement data, which were expressed by mean ± standard deviation. The experiment was independently repeated 3 times. * p < 0.05.