Nucleic acid-binding property of CSDP1 and GRP7

Copyright information:Taken from "Cold shock domain proteins and glycine-rich RNA-binding proteins from can promote the cold adaptation process in "Nucleic Acids Research 2006;35(2):506-516.Published online 14 Dec 2006PMCID:PMC1802614.© 2006 The Author(s) () The -synthesized S-labeled proteins were mixed with ssDNA, dsDNA (calf thymus) and four kinds of ribonucleotide homopolymers bound to agarose beads at 250 mM NaCl. Bound proteins were separated by SDS–PAGE, and the protein bands were visualized by an Image analyzer. () SELEX was performed using recombinant GRP7–GST protein and -transcribed RNAs with 65 nt-long randomized regions. The average occurrence of each nucleotide in 30 different RNA sequences is represented.